北京荣志海达最新引进奥地利BIOMEDICA公司生产的可溶性总神经纤毛蛋白-1检测试剂盒(酶联免疫法)，欢迎广大科研用户前来咨询。
可溶性总神经纤毛蛋白-1检测试剂盒(酶联免疫法)
Total Soluble Neuropilin-1 ELISA
全球首家推出由表位定位特异性抗体定量检测
nNrp-1在神经系统中与其配体 semaphorins (信号素）结合而在轴突中起作用， Nrp-1， Nrp-2为 Sema受体并且仅与III型Sema结合。
应用领域：骨学，肿瘤学，肾脏学，心血管。
试剂盒信息
试剂盒信息
|
货号 |
BI-20409 |
|
方法学 |
夹心 ELISA, HRP/TMB, 12x8人份测试 |
|
样本类型 |
血清、血浆(EDTA, 肝素,枸橼酸钠)、细胞培养液 |
|
标准品范围 |
0-12 nmol/l (7个标准品） |
|
标准品 |
0/0.37/0.75/1.5/3/6/12 nmol/l |
|
质控品 |
2个 |
|
样本量 |
10 μl /测试 |
|
孵育时间 |
30 min/2h/1h/30min |
|
单位换算 |
1ng/ml = 0.014nmol/l (MW: 69.7 kDa) |
|
灵敏度 |
LOD: 0.09 nmol/l (0 pmol/l + 3 SD); LLOQ: 0.09nmol/l |
|
精密度 |
批内差 (n=6) ≤ 11%, 批间差 (n=12) ≤ 10% |
|
健康人群参考范围 |
Median 血清( n=24): 2.0nmol/l. Median EDTA 血浆(n=24): 1.7nmol/l. Median 肝素 血浆(n=24): 1.7nmol/l. Median枸橼酸钠血浆(n=24):
1.9nmol/l. |
|
非人属种Nrp-1 |
猴子，猪，大鼠及小鼠与人Nrp-1同源性超过90%，本试剂并未对非人属种样本做验证，但是大鼠及小鼠的加标回收率以及线性数据表现合格。
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参考文献
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3. Characterization of neuropilin-1 structural features that confer binding to semaphorin 3A and vascular endothelial growth factor 165. Gu C et al., J Biol Chem, 2002; 277(20): 18069-18076.
4. Multifaceted Role of Neuropilins in the Immune System: Potential Targets for Immunotherapy. Roy S et al., Front Immunol, 2017; 10 (8): 1228.
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6. Role of Neuropilin-1 in Diabetic Nephropathy. Bondeva T et al., J Clin Med, 2015; 4(6): 1293-1311.
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8. Neuropilin-1 is upregulated in the adaptive response of prostate tumors to androgen-targeted therapies and is prognostic of metastatic progression and patient mortality. Tse BWC et al., Oncogene, 2017; 36(24): 3417-3427.
9. Dual Function of NRP1 in Axon Guidance and Subcellular Target Recognition in Cerebellum. Telley L et al., Neuron, 2016; 21; 91(6): 1276-1291.
10. Nrp1, a Neuronal Regulator, Enhances DDR2-ERK-Runx2 Cascade in Osteoblast Differentiation via Suppression of DDR2 Degradation. Zhang Y et al., Cell Physiol Biochem, 2015; 36(1): 75-84.
11.Neuropilins: structure, function and role in disease. Pellet-Many C et al., Biochem J, 2008; 411(2): 211-226.
12. Neuropilin-1 as Therapeutic Target for Malignant Melanoma. G Graziani1 and PM Lacal, Front Oncol, 2015; 5: 125.
13. CPMP/ICH/381/95 ICH Topic Q2 (R1) „Validation of Analytical Procedures: Text and Methodology” including: ICH Q2A “Text on Validation of Analytical Procedures” ICH Q2B “Validation of Analytical Procedures: Methodology”.
14.Neuropilins lock secreted semaphorins ontoplexins in a ternary signaling complex. Janssen BJC et al., Nat struct Mol Biol, 2012; 19:1293-1299.