Enzyme immunoassay kit for the quantitative determination of Vitamin-D3 in dairy samples. For in vitro quantification use only.
I. PROPRIETARY NAME
The VitaKit DTM contains sufficient material to assay 96 tests.
II. APPLICATION AND INTENDED USE
Dairy milk is fortified with vitamins A & D3, as milk has become the major source of these vitamins for human beings. Regulatory agencies have set standards specifying the amount of vitamins A and D3 to be added to milk products. The methodology has been designed to extract the vitamins from milk fat, and to directly quantify the amount of vitamins in an ELISA based assay. Other methods that can detect vitamins in dairy milk are time consuming and require specialised laboratory equipment and trained personnel.
The VitaKit DTM provides materials for the quantitative measurement of vitamin-D3 in dairy products. This assay is intended for in vitro quantification only.
III. PRINCIPLES OF THE METHOD
The Vitamin D3 ELISA test is based on the principle of a competitive enzyme immunoassay. The assay system utilizes a fixed number of Vitamin D3 molecules immobilized on a solid phase. These molecules compete with an unknown number of Vitamin D3 molecules extracted from milk samples for a fixed number of binding sites on enzyme-labelled monoclonal antibodies directed against the Vitamin D3. As the number of Vitamin D3 molecules in the sample increases, the number of bound labelled antibody molecules to solid phase antigen decreases due to competition. The amount of enzyme-labelled antibodies bound to the solid phase Vitamin D3 is inversely proportional to the concentration of Vitamin D3 present in the sample.
