Assay characteristics:
| Cat.No.: | BI-20492 |
| Method: | Sandwich ELISA, HRP/TMB, 12x8-well strips |
| Sample type: | Serum, plasma (EDTA, heparin), urine protocol available |
| Standard range: | 0-240 pmol/l (6 serum based standards) |
| Standard points: | 0/15/30/60/120/240 pmol/l |
| Control: | 1 serum based control |
| Sample size: | 20 μl / well |
| Incubation time: | Overnight / 1 h / 30 min |
| Unit conversion: | 1 pg/ml = 0.044 pmol/l (MW: 22.5 kDa) |
Sensitivity:
LOD: 3.2 pmol/l (0 pmol/l + 3 SD); LLOQ: < 7.5 pmol/l
Precision:
Intra-assay (n=8) ≤ 7%, Inter-assay (n=6) ≤ 10%
Spike/Recovery:
The mean recovery of recombinant Sclerostin in human serum samples (n=6) is 94%.
Dilution linearity:
Endogenous Sclerostin:
The mean dilution linearity of endogenous Sclerostin in human serum samples (n=4) is 110% for dilution factors 1+1, 1+3, 1+7.
Recombinant Sclerostin:
The mean dilution linearity for recombinant Sclerostin in human serum samples (n=3) is 98% for dilution factors 1+1, 1+3.
Cross reactivity:
The assay does not detect Noggin.
The assay does not detect Wise (SOSTDC1).
Species cross reactivity:
Rat, mouse: The assay does not deted rat or mouse Sclerostin.
Primates: The sequence homology of human Sclerostin to various primate species is >95%. It is likely that the assay can be used for these species. Internal validations have not been carried out. However, data from other laboratories show positive results.
Serum values from apparently healthy individuals:
Median (serum, n=411): 24.14. pmol/l.
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!